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Rev. méd. Chile ; 140(4): 476-483, abr. 2012. ilus
Article in Spanish | LILACS | ID: lil-643217

ABSTRACT

Background: Entamoeba histolytica and Entamoeba dispar are morphologically identical. However, the former is highly pathogenic and the latter is not. Aim: To differentiate Entamoeba histolytica from Entamoeba dispar through ELISA and PCR techniques in Colombian isolates from feces. Material and Methods: Descriptive study of Colombian fecal samples from 53 males and 47 women, that were positive for the complex E. histolytica/E. dispar on light microscopy. Positive samples were cultured on Robinson medium to isolate trophozoites. The presence of specific Gal/ GalNAc-lectin was determined by ELISA and polymerase chain reaction in genomic DNA, using the combination of three nucleotides that recognize a variable region of 16S small subunit ribosomal RNA, generating a 166 base pair (bp) product for E. histolytica and 752 pb product for E. dispar. Results: After verification, only eight of the 100 samples were positive for the complex E. histolytica/E. dispar and were cultivated. Isolates were obtained in six cultures, one corresponded to E. histolytica and six to E. dispar. Conclusions: The presence of E. histolytica/E. dispar complex was largely overestimated with light microscopy. In the few samples where isolates were obtained, the technique described differentiated between both strains.


Subject(s)
Female , Humans , Male , Entamoeba/metabolism , Entamoebiasis/parasitology , Colombia , DNA, Protozoan/genetics , Entamoeba histolytica/genetics , Entamoeba histolytica/isolation & purification , Entamoeba/genetics , Entamoeba/isolation & purification , Entamoebiasis/diagnosis , Enzyme-Linked Immunosorbent Assay , Feces/parasitology , Lectins , Polymerase Chain Reaction/methods , Protozoan Proteins , /genetics , Sensitivity and Specificity
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